Blatant Biology

digestive enzyme found in pancreatic juice of the duodenum, catalysing proteolysis; cleaves proteins on the N-terminal of large aliphatic amino acids (tyrosine, tryptophan, phenylalanine)

serine protease that breaks down elastin

a set of three coordinated amino acids found within the active site of some enzymes; can be conserved across classes of enzymes (including spatial conservation)

proportion of amino acids that are identical between two protein sequences

a measure of how closely related two amino acid sequences are, by class of amino acid (hydrophobic, hydrophilic, ...)

highly sequence-specific protease used for the controlled cleavage of fusion proteins

blood coagulation factor and sequence-specific serine protease; less specific than TEV, and can cleave almost any protein if incubated for long enough

a technique to separate biomolecules through the selective binding of a molecule, and then eluting the column to release the molecule of interest; the process used in the practical session

an inactive form of chymotrypsin, which gets cleaved to produce the active form

precursor form of trypsin, produced in the pancreas

inactive precursor form of elastase, produced by the acinar cells of the pancreas

a serine protease obtained from Bacillus subtilis

anti-cancer drug inhibiting the 26S proteasome, with high specificity and affinity; the boron atom transitions between trigonal and tetrahedral geometries